Journal: STAR Protocols

Article Title: An optimized protocol for phenotyping human granulocytes by mass cytometry

doi: 10.1016/j.xpro.2022.101280

Figure Lengend Snippet:

Article Snippet: Paired bone marrow and peripheral blood from healthy donors , AllCells , https://www.allcells.com/.

Techniques: Recombinant, Blocking Assay, Electron Microscopy, Red Blood Cell Lysis, Antibody Labeling, Membrane, Software

Journal: Leukemia

Article Title: Targeting IL-17A in Multiple Myeloma: A Potential Novel Therapeutic Approach in Myeloma

doi: 10.1038/leu.2015.228

Figure Lengend Snippet: The MM patient samples were collected after informed consent in accordance with the Declaration of Helsinki and approved by the institutional review board (IRB) from Dana-Farber Cancer Institute. Healthy donor bone marrow samples were obtained from AllCells (Emeryville, CA). MM primary cells were purified as described earlier5. RNA was isolated from MM cell-lines (A) and purified MM primary cells (B). Quantitative PCR was performed for IL-17A using 7900HT from Applied Biosystems. Representative experimental results from three different experiments were presented as relative expression value in comparison with GAPDH. For immuno-blot experiments, total cell lysates were prepared from MM cell-lines (C) and purified CD138+ MM primary cells (D), and separated by electrophoresis on 5% to 20% polyacrylamide gradient gels. Samples were probed with anti-sera to IL-17A and GAPDH as indicated. Representative immunoblot of three different experiments was shown. Myeloma cell lines were stained with isotype control antibody or anti–IL-17A antibody and analyzed by confocal microscopy. One representative cell line of 4 experiments was shown at 640 magnifications (E).

Article Snippet: Healthy donor bone marrow samples were obtained from AllCells (Emeryville, CA).

Techniques: Purification, Isolation, Real-time Polymerase Chain Reaction, Expressing, Comparison, Electrophoresis, Western Blot, Staining, Control, Confocal Microscopy